Delineating the specificity of an IgE-encoding transcriptome.

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Delineating the specificity of an IgE-encoding transcriptome. / Persson, Helena; Sadegh, Mardjaneh Karbalaei; Greiff, Lennart; Ohlin, Mats.

In: Journal of Allergy and Clinical Immunology, Vol. 120, No. 5, 2007, p. 1186-1192.

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Persson, Helena ; Sadegh, Mardjaneh Karbalaei ; Greiff, Lennart ; Ohlin, Mats. / Delineating the specificity of an IgE-encoding transcriptome. In: Journal of Allergy and Clinical Immunology. 2007 ; Vol. 120, No. 5. pp. 1186-1192.

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TY - JOUR

T1 - Delineating the specificity of an IgE-encoding transcriptome.

AU - Persson, Helena

AU - Sadegh, Mardjaneh Karbalaei

AU - Greiff, Lennart

AU - Ohlin, Mats

PY - 2007

Y1 - 2007

N2 - Background Although much is known about the reactivity of polyclonal populations of antibodies targeting the wide array of allergens produced by timothy (Phleum pratense) and other grass species, little is known about the finer details at the level of individual antibody specificities. Objective We sought to investigate the IgE repertoire as it occurs in a patient with grass pollen allergy. Methods For this purpose, a human IgE library was used, constructed from peripheral blood B cells of an individual with timothy allergy. The library was screened by using phage display against a panel of 6 timothy allergens (Phl p 1, Phl p 2, Phl p 4, Phl p 5, Phl p 6, and Phl p 11). Results Highly diverse antibody fragments with respect to gene usage were identified. The binders were specific for their respective target antigen, except for clones selected on Phl p 6 that also recognized Phl p 5, most likely reflecting the high sequence homology between these allergens. Interestingly, by using this approach, we were able to determine the specificity of more than 25% of all IgE-producing transcripts in this individual with allergy. Conclusion The human IgE repertoire is produced by a limited number of highly related B-cell clones and as such is restricted in its recognition of a limited number of antigens. Clinical implications Human allergen-specific antibodies can, by defining the specificity of IgE responses, aid in the development of allergy vaccines or even by themselves be used in passive immunotherapy.

AB - Background Although much is known about the reactivity of polyclonal populations of antibodies targeting the wide array of allergens produced by timothy (Phleum pratense) and other grass species, little is known about the finer details at the level of individual antibody specificities. Objective We sought to investigate the IgE repertoire as it occurs in a patient with grass pollen allergy. Methods For this purpose, a human IgE library was used, constructed from peripheral blood B cells of an individual with timothy allergy. The library was screened by using phage display against a panel of 6 timothy allergens (Phl p 1, Phl p 2, Phl p 4, Phl p 5, Phl p 6, and Phl p 11). Results Highly diverse antibody fragments with respect to gene usage were identified. The binders were specific for their respective target antigen, except for clones selected on Phl p 6 that also recognized Phl p 5, most likely reflecting the high sequence homology between these allergens. Interestingly, by using this approach, we were able to determine the specificity of more than 25% of all IgE-producing transcripts in this individual with allergy. Conclusion The human IgE repertoire is produced by a limited number of highly related B-cell clones and as such is restricted in its recognition of a limited number of antigens. Clinical implications Human allergen-specific antibodies can, by defining the specificity of IgE responses, aid in the development of allergy vaccines or even by themselves be used in passive immunotherapy.

KW - antibody library

KW - Allergy

KW - antibody repertoire

KW - diversity

KW - grass pollen allergen

KW - IgE

KW - specificity

KW - phage display

U2 - 10.1016/j.jaci.2007.06.041

DO - 10.1016/j.jaci.2007.06.041

M3 - Article

VL - 120

SP - 1186

EP - 1192

JO - Journal of Allergy and Clinical Immunology

T2 - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 1097-6825

IS - 5

ER -