Dendritic cell KLF2 expression regulates T cell activation and proatherogenic immune responses

Research output: Contribution to journalArticle

Abstract

Dendritic cells (DCs) have been implicated as important regulators of innate and adaptive inflammation in many diseases, including atherosclerosis. However, the molecular mechanisms by which DCs mitigate or promote inflammatory pathogenesis are only partially understood. Previous studies have shown an important anti-inflammatory role for the transcription factor Krüppel-like factor 2 (KLF2) in regulating activation of various cell types that participate in atherosclerotic lesion development, including endothelial cells, macrophages, and T cells. We used a pan-DC, CD11c-specific cre-lox gene knockout mouse model to assess the role of KLF2 in DC activation, function, and control of inflammation in the context of hypercholesterolemia and atherosclerosis. We found that KLF2 deficiency enhanced surface expression of costimulatory molecules CD40 and CD86 in DCs and promoted increased T cell proliferation and apoptosis. Transplant of bone marrow from mice with KLF2-deficient DCs into Ldlr-/- mice aggravated atherosclerosis compared with control mice, most likely due to heightened vascular inflammation evidenced by increased DC presence within lesions, enhanced T cell activation and cytokine production, and increased cell death in atherosclerotic lesions. Taken together, these data indicate that KLF2 governs the degree of DC activation and hence the intensity of proatherogenic T cell responses.

Details

Authors
  • Noah Alberts-Grill
  • Daniel Engelbertsen
  • Dexiu Bu
  • Amanda Foks
  • Nir Grabie
  • Jan M. Herter
  • Felicia Kuperwaser
  • Tao Chen
  • Gina Destefano
  • Petr Jarolim
  • Andrew H. Lichtman
External organisations
  • Brigham and Women's Hospital, Boston
Original languageEnglish
Pages (from-to)4651-4662
JournalJournal of Immunology
Volume197
Issue number12
Publication statusPublished - 2016 Dec 15
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes