Dependence of macrophage superoxide release on the pulse amplitude of an applied pressure regime: A potential factor at the soft tissue-implant interface

Research output: Contribution to journalArticle


Failure of soft tissue implants has been largely attributed to the influence of biomaterial surface properties on the foreign body response, but some implant complications, e.g. macrophage accumulation and necrosis, are still not effectively addressed with surface treatments to minimize deleterious biomaterial effects. We explored an alternative explanation for implant failure, linking biocompatibility with implant micromotion-induced pressure fluctuations at the tissue-biomaterial interface. For this purpose, we used a custom in vitro system to characterize the effects of pressure fluctuations on the activity of macrophages, the predominant cells at a healing implant site. Initially, we quantified superoxide production by HL60-derived macrophage-like cells under several different pressure regimes with means of 5-40 mmHg, amplitudes of 0-15 mmHg and frequencies of 0-1.5 Hz. All pressure regimes tested elicited significantly (p2=0.74; p


  • Hainsworth Y. Shin
  • Danielle M. Frechette
  • Nathan Rohner
  • Xiaoyan Zhang
  • David A. Puleo
  • Lars M. Bjursten
External organisations
  • University of Kentucky
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Other Clinical Medicine
  • Other Basic Medicine


  • Cell adhesion, Macrophage, Mechanotransduction, Pressure, Superoxide, Tissue-implant interface
Original languageEnglish
Pages (from-to)E227-E238
JournalJournal of Tissue Engineering and Regenerative Medicine
Issue number3
Publication statusPublished - 2016 Mar 1
Publication categoryResearch