Design of high-density antibody microarrays for disease proteomics: Key technological issues.

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Abstract

Antibody-based microarray is a novel proteomic technology setting a new standard for molecular profiling of non-fractionated complex proteomes. The first generation of antibody microarrays has already demonstrated its potential for generating detailed protein expression profiles, or protein atlases, of human body fluids in health and disease, paving the way for new discoveries within the field of disease proteomics. The process of designing highly miniaturized, high-density and high-performing antibody microarray set-ups have, however, proven to be challenging. In this mini-review we discuss key technological issues that must be addressed in a cross-disciplinary manner before true global proteome analysis can be performed using antibody microarrays.

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  • Immunology in the medical area
Original languageEnglish
Pages (from-to)928-935
JournalJournal of Proteomics
Volume72
Issue number6
Publication statusPublished - 2009
Publication categoryResearch
Peer-reviewedYes