Detailed characterization of plasmids carrying resistance genes using optical DNA mapping

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceeding

Abstract

We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.

Details

Authors
  • Vilhelm Müller
  • Lena K. Nyberg
  • Saair Quaderi
  • Erik Kristiansson
  • Tobias Ambjörnsson
  • Linus Sandegren
  • Fredrik Westerlund
Organisations
External organisations
  • Chalmers University of Technology
  • Uppsala University
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Laboratory and Measurements Technologies

Keywords

  • Antibiotic resistance, CRISPR/Cas9, Nanofluidics, Optical DNA mapping, Plasmids
Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages1561-1562
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Publication categoryResearch
Peer-reviewedYes
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period2016/10/092016/10/13