Detection of Bax Microsatellite Mutations and BaxΔ2 Isoform in Human Buccal Cells

Research output: Contribution to journalArticle

Abstract

Loss of the pro-apoptotic Bcl-2 family protein Bax occurs in ~50% of hereditary nonpolyposis colorectal cancer (HNPCC) due to microsatellite instability (MSI). Recently, we found that some of the "Bax-negative" MSI tumor cells contain a functional Bax isoform, BaxΔ2, which sensitizes cells to selective chemotherapeutics. Here we show the detection of Bax microsatellite mutations and expression of BaxΔ2 proteins in human buccal cells. Our study provides a sensitive and non-invasive approach and a potential clinical application in diagnosis and treatment of MSI colon cancer patients.

Details

Authors
  • Honghong Zhang
  • Cecilie Tassone
  • Nora Lin
  • Adriana Mañas
  • Yu Zhao
  • Jialing Xiang
External organisations
  • Illinois Institute of Technology
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology
Original languageEnglish
Article number002
JournalJournal of cell science & therapy
Volume6
Issue numberSuppl 8
Publication statusPublished - 2015 Sep
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes