Detection of Helicobacter species in chronic liver disease and chronic inflammatory bowel disease

Research output: Contribution to journalArticle

Abstract

Aim. To study the association between helicobacters and chronic liver diseases and chronic inflammatory bowel diseases. Patients and methods. Thirty-two patients with various chronic liver diseases and 137 patients with inflammatory bowel disease were enrolled. Antibodies to H. pylori, H. hepaticus, H. bilis, and H. pullorum were measured by enzyme immunoassay (EIA), and sera positive in a non-pylori helicobacter EIA were further examined by immunoblot assay. Detection of Helicobacter DNA in liver biopsies was done by denaturating gradient gel electrophoresis of PCR products (PCR-DGGE) and DNA sequence analysis. Results. Six inflammatory bowel disease patients, four with ulcerative colitis and two with Crohn's disease, and one liver disease patient with autoimmune cholangitis had antibodies to non-pylori helicobacters by an immunoblot assay. Four immunoblot assay-negative patients, three with autoimmune and one with non-autoimmune liver disease, had Helicobacter DNA in liver biopsies; three of the polymerase chain reaction (PCR) products were closely related to non-pylori helicobacters. Conclusion. Evidence for non-pylori helicobacters was scant in Finnish patients with inflammatory bowel disease or chronic but not end stage liver disease. We cannot, however, rule out their role in these diseases.

Details

Authors
  • Lea Veijola
  • Ingrid Nilsson
  • Leena Halme
  • Waleed Abu Al-Soud
  • Judit Makinen
  • Åsa Ljungh
  • Hilpi Rautelin
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area

Keywords

  • blotting, Western, PCR, liver disease, Helicobacter, inflammatory bowel disease
Original languageEnglish
Pages (from-to)554-560
JournalAnnals of Medicine
Volume39
Issue number7
Publication statusPublished - 2007
Publication categoryResearch
Peer-reviewedYes