Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.

Research output: Contribution to journalArticle

Abstract

Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.

Details

Authors
  • D. Owerbach
  • A. Lernmark
  • L. Rask
  • P. A. Peterson
  • P. Platz
  • A. Svejgaard
External organisations
  • Hagedorn Research Institute
Original languageEnglish
Pages (from-to)3758-3761
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number12
Publication statusPublished - 1983 Jan 1
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes