Deubiquitination of γ-tubulin by BAP1 prevents chromosome instability in breast cancer cells.

Research output: Contribution to journalArticle

Abstract

Microtubule nucleation requires the γ-tubulin ring complex, and during the M phase (mitosis) this complex accumulates at the centrosome to support mitotic spindle formation. The post-translational modification of γ-tubulin through ubiquitination is vital for regulating microtubule nucleation and centrosome duplication. Blocking the BRCA1/BARD1-dependent ubiquitination of γ-tubulin causes centrosome amplification. In the present study, we identified BRCA1 associated protein-1 (BAP1) as a deubiquitination enzyme for γ-tubulin. BAP1 was downregulated in metastatic adenocarcinoma breast cell lines compared to non-cancerous human breast epithelial cells. Furthermore, low expression of BAP1 was associated with reduced overall survival of breast cancer patients. Reduced expression of BAP1 in breast cancer cell lines was associated with mitotic abnormalities. Importantly, rescue experiments including expression of full length but not the catalytic mutant of BAP1 reduced ubiquitination of γ-tubulin and prevented mitotic defects. Our study uncovers a new mechanism for BAP1 involved in deubiquitination of γ-tubulin, which is required to prevent abnormal mitotic spindle formation and genome instability.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology
Original languageEnglish
Pages (from-to)6499-6508
JournalCancer Research
Volume74
Issue number22
Publication statusPublished - 2014
Publication categoryResearch
Peer-reviewedYes

Related research output

Zarrizi, R., 2015, Division of Translational Cancer Research. 49 p.

Research output: ThesisDoctoral Thesis (compilation)

View all (1)