Development of an automated flow-based spectrophotometric immunoassay for continuous detection of zearalenone

Research output: Contribution to journalArticle

Abstract

Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time- and cost-effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an automated sequential injection system. Time for an assay cycle was 18 Min. Under optimal conditions, the limit for quantification for ZEN was 0.40 µg L−1 with a linear dependency between concentration and signal amplitude between 0.10 and 10 µg L−1. The assay proved to be robust and reliable with 13% relative standard deviation between measurements. By dissociating the antigen–antibody complex using a regeneration solution, we showed 50 times reusability of the immobilized antibodies without affecting the antigen-binding properties.

Details

Authors
Organisations
External organisations
  • King Mongkut's Institute of Technology Ladkrabang
  • Technical University of Denmark
  • Ghent University
  • CapSenze Biosystems AB
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • bioanalysis, flow-ELISA, immunoassay, mycotoxin, sequential injection, zearalenone
Original languageEnglish
Pages (from-to)375-382
Number of pages8
JournalBiotechnology and Applied Biochemistry
Volume67
Issue number3
Publication statusPublished - 2020 May 1
Publication categoryResearch
Peer-reviewedYes