Development of Novel Diffusion NMR Methods: Preclinical Applications in Colloidal Model Systems

Research output: ThesisDoctoral Thesis (compilation)

Standard

Development of Novel Diffusion NMR Methods : Preclinical Applications in Colloidal Model Systems. / Eriksson, Stefanie.

Lund : Lund University, Faculty of Science, Center for Chemistry and Chemical Engineering, 2016. 131 p.

Research output: ThesisDoctoral Thesis (compilation)

Harvard

APA

Eriksson, S. (2016). Development of Novel Diffusion NMR Methods: Preclinical Applications in Colloidal Model Systems. Lund: Lund University, Faculty of Science, Center for Chemistry and Chemical Engineering.

CBE

Eriksson S. 2016. Development of Novel Diffusion NMR Methods: Preclinical Applications in Colloidal Model Systems. Lund: Lund University, Faculty of Science, Center for Chemistry and Chemical Engineering. 131 p.

MLA

Eriksson, Stefanie Development of Novel Diffusion NMR Methods: Preclinical Applications in Colloidal Model Systems Lund: Lund University, Faculty of Science, Center for Chemistry and Chemical Engineering. 2016.

Vancouver

Eriksson S. Development of Novel Diffusion NMR Methods: Preclinical Applications in Colloidal Model Systems. Lund: Lund University, Faculty of Science, Center for Chemistry and Chemical Engineering, 2016. 131 p.

Author

Eriksson, Stefanie. / Development of Novel Diffusion NMR Methods : Preclinical Applications in Colloidal Model Systems. Lund : Lund University, Faculty of Science, Center for Chemistry and Chemical Engineering, 2016. 131 p.

RIS

TY - THES

T1 - Development of Novel Diffusion NMR Methods

T2 - Preclinical Applications in Colloidal Model Systems

AU - Eriksson, Stefanie

N1 - Defence details Date: [2016-05-27] Time: [13:15] Place: [The Center for chemistry and chemical engineering, lecture hall A, Naturvetarvägen 14 (former Getingevägen 60), Lund] External reviewer Name: [Ronen, Itamar] Title: [Dr.] Affiliation: [Leiden University Medical Center, Leiden, The Netherlands]

PY - 2016

Y1 - 2016

N2 - NMR is a powerful non-invasive technique utilizing radiofrequency and gradient pulses to manipulate the spinsof atomic nuclei. In particular, diffusion NMR uses strong magnetic field gradients to label the positions of spins allowing for the measurement of translational motion of molecules in liquids. The average displacement of water molecules can give information on the pore microstructure in various materials including biological cells and tissues.The interpretation of conventional diffusion NMR signal can be ambiguous, as it contains effects from microstructure, size and orientation. In this work, the underlying microscopic structures are exposed with novel diffusion NMR methods. q-MAS is a pulse sequence for isotropic diffusion encoding, which in combination with conventional diffusion NMR can be used to quantify the microscopic anisotropy. The shape of the microscopic diffusion tensor can be revealed by varying the anisotropy of the diffusion encoding. The methods are demonstrated in lyotropic liquid crystals and yeast cell suspensions.Diffusion NMR can be used to measure exchange between intra- and extracellular compartments. This can give information on the water transport across biological cell membranes. A method for measuring exchange in yeast cells with different intra- and extracelllular T2 values is presented.

AB - NMR is a powerful non-invasive technique utilizing radiofrequency and gradient pulses to manipulate the spinsof atomic nuclei. In particular, diffusion NMR uses strong magnetic field gradients to label the positions of spins allowing for the measurement of translational motion of molecules in liquids. The average displacement of water molecules can give information on the pore microstructure in various materials including biological cells and tissues.The interpretation of conventional diffusion NMR signal can be ambiguous, as it contains effects from microstructure, size and orientation. In this work, the underlying microscopic structures are exposed with novel diffusion NMR methods. q-MAS is a pulse sequence for isotropic diffusion encoding, which in combination with conventional diffusion NMR can be used to quantify the microscopic anisotropy. The shape of the microscopic diffusion tensor can be revealed by varying the anisotropy of the diffusion encoding. The methods are demonstrated in lyotropic liquid crystals and yeast cell suspensions.Diffusion NMR can be used to measure exchange between intra- and extracellular compartments. This can give information on the water transport across biological cell membranes. A method for measuring exchange in yeast cells with different intra- and extracelllular T2 values is presented.

KW - diffusion NMR

KW - diffusion tensor

KW - Microscopic anisotropy

KW - q-MAS

KW - exchange

KW - liquid crystals

KW - yeast cell suspensions

M3 - Doctoral Thesis (compilation)

SN - 978-91-7623-824-0

PB - Lund University, Faculty of Science, Center for Chemistry and Chemical Engineering

CY - Lund

ER -