Development of polymorphic nuclear microsatellite markers for polyploid and diploid members of the orchid genus Dactylorhiza

Research output: Contribution to journalArticle

Abstract

Dactylorhiza traunsteineri is an allotetraploid species that belongs to the large Dactylorhiza incarnata/maculata polyploid complex of the Eurasian genus Dactylorhiza (Orchidaceae). Here, eight polymorphic microsatellite loci were isolated using selective hybridization according to the FIASCO protocol (fast isolation by AFLP of sequences containing repeats) with slight modifications. The number of alleles per locus ranged from two to seven. All loci were possible to amplify in several other species of Dactylorhiza, using the same primers.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Ecology

Keywords

  • narrowed-leaved marsh orchid, microsatellites, cross-species amplification, Dactylorhiza, polymorphism, polyploidy
Original languageEnglish
Pages (from-to)644-647
JournalMolecular Ecology Notes
Volume7
Issue number4
Publication statusPublished - 2007
Publication categoryResearch
Peer-reviewedYes