Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning

Research output: Contribution to journalArticle

Abstract

We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • precursor-ion, reagents, mass spectrometry, isotope labeling, HPLC, parent-ion
Original languageEnglish
Pages (from-to)1101-1113
JournalJournal of Proteome Research
Volume6
Issue number3
Publication statusPublished - 2007
Publication categoryResearch
Peer-reviewedYes