Different expression levels of glycans on leukemic cells—a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

Research output: Contribution to journalArticle


Sialic acid (SA) is normally expressed on the cell membranes and is located at the terminal position of the sugar chains. SA plays an important role for regulation of the innate immunity, function as markers of the cells and can be recognized by a variety of receptors. Interestingly, the level of SA expression is increased on metastatic cancer cells. The availability of specific antibodies against SA is limited and, therefore, biomarker tools for detection of SA are lacking. We have recently presented a novel method for specific fluorescence labeling of SA molecular imprinted polymers (MIP). Here, we have performed an extended screening of SA expression by using SA-MIP and included four different chronic lymphocytic leukemia (CLL) cell lines, conveniently analyzed by flow cytometry and fluorescence microscopy. SA expression was detected in four cell lines at different levels, and the SA expression were verified with lectin-FITC. These results show that SA-MIP can be used as a plastic antibody for detection of SA using both flow cytometry and fluorescence microscopy. We suggest that SA-MIP can be used for screening of different tumor cells of various stages, including CLL cells.


  • Zahra El-Schich
  • Mohammad Abdullah
  • Sudhirkumar Shinde
  • Nishtman Dizeyi
  • Anders Rosén
  • Börje Sellergren
  • Anette Gjörloff Wingren
External organisations
  • Malmö University
  • Linköping University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology


  • Chronic lymphocytic leukemia, Lectin, Molecular imprinting polymers, Sialic acid
Original languageEnglish
Pages (from-to)13763-13768
JournalTumor Biology
Issue number10
Publication statusPublished - 2016
Publication categoryResearch