Direct colorimetric assay for rapid detection of rifampin-resistant Mycobacterium tuberculosis

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Direct colorimetric assay for rapid detection of rifampin-resistant Mycobacterium tuberculosis. / Abate, Getahun; Aseffa, Abraham; Selassie, Alemayehu; Goshu, Solomon; Fekade, Bekele; WoldeMeskal, Dawit; Miörner, Håkan.

In: Journal of Clinical Microbiology, Vol. 42, No. 2, 2004, p. 871.

Research output: Contribution to journalArticle

Harvard

Abate, G, Aseffa, A, Selassie, A, Goshu, S, Fekade, B, WoldeMeskal, D & Miörner, H 2004, 'Direct colorimetric assay for rapid detection of rifampin-resistant Mycobacterium tuberculosis', Journal of Clinical Microbiology, vol. 42, no. 2, pp. 871. https://doi.org/10.1128/JCM.42.2.871-873.2004

APA

Abate, G., Aseffa, A., Selassie, A., Goshu, S., Fekade, B., WoldeMeskal, D., & Miörner, H. (2004). Direct colorimetric assay for rapid detection of rifampin-resistant Mycobacterium tuberculosis. Journal of Clinical Microbiology, 42(2), 871. https://doi.org/10.1128/JCM.42.2.871-873.2004

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MLA

Vancouver

Author

Abate, Getahun ; Aseffa, Abraham ; Selassie, Alemayehu ; Goshu, Solomon ; Fekade, Bekele ; WoldeMeskal, Dawit ; Miörner, Håkan. / Direct colorimetric assay for rapid detection of rifampin-resistant Mycobacterium tuberculosis. In: Journal of Clinical Microbiology. 2004 ; Vol. 42, No. 2. pp. 871.

RIS

TY - JOUR

T1 - Direct colorimetric assay for rapid detection of rifampin-resistant Mycobacterium tuberculosis

AU - Abate, Getahun

AU - Aseffa, Abraham

AU - Selassie, Alemayehu

AU - Goshu, Solomon

AU - Fekade, Bekele

AU - WoldeMeskal, Dawit

AU - Miörner, Håkan

PY - 2004

Y1 - 2004

N2 - The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was standardized for direct detection of rifampin-resistant Mycobacterium tuberculosis in sputum samples. The sensitivity and specificity of the direct MTT assay matched those of the standard indirect susceptibility assay on 7H10 medium, and interpretable results were obtained for 98.5% of the samples within 2 weeks. Traditional methods of in vitro drug susceptibility testing are time consuming and laborious. Susceptibility tests on clinical isolates require 6 to 9 weeks, and tests conducted directly on smear-positive samples take about 3 weeks (International Union Against Tuberculosis and Lung Disease, The public health service national tuberculosis reference laboratory and the national laboratory network. Minimum requirements, role and operation in a low-income country, Paris, France, 1998, and P. T. Kent and G. P. Kubica, Public health mycobacteriology. A guide for the level III laboratory, Centers for Disease Control and Prevention, Atlanta, Ga., 1985). More-rapid methods are available but are very expensive for routine use under program conditions in countries with high levels of tuberculosis endemicity.

AB - The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was standardized for direct detection of rifampin-resistant Mycobacterium tuberculosis in sputum samples. The sensitivity and specificity of the direct MTT assay matched those of the standard indirect susceptibility assay on 7H10 medium, and interpretable results were obtained for 98.5% of the samples within 2 weeks. Traditional methods of in vitro drug susceptibility testing are time consuming and laborious. Susceptibility tests on clinical isolates require 6 to 9 weeks, and tests conducted directly on smear-positive samples take about 3 weeks (International Union Against Tuberculosis and Lung Disease, The public health service national tuberculosis reference laboratory and the national laboratory network. Minimum requirements, role and operation in a low-income country, Paris, France, 1998, and P. T. Kent and G. P. Kubica, Public health mycobacteriology. A guide for the level III laboratory, Centers for Disease Control and Prevention, Atlanta, Ga., 1985). More-rapid methods are available but are very expensive for routine use under program conditions in countries with high levels of tuberculosis endemicity.

U2 - 10.1128/JCM.42.2.871-873.2004

DO - 10.1128/JCM.42.2.871-873.2004

M3 - Article

VL - 42

SP - 871

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 1098-660X

IS - 2

ER -