Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ 51]CR-release

Research output: Contribution to journalArticle

Standard

Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ 51]CR-release. / Kromann, H.; Christy, M.; Egeberg, J.; Lernmark, A.; Nerup, J.; Richter-Olesen, H.

In: Medical Biology, Vol. 58, No. 6, 01.12.1980, p. 322-328.

Research output: Contribution to journalArticle

Harvard

Kromann, H, Christy, M, Egeberg, J, Lernmark, A, Nerup, J & Richter-Olesen, H 1980, 'Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ 51]CR-release', Medical Biology, vol. 58, no. 6, pp. 322-328.

APA

Kromann, H., Christy, M., Egeberg, J., Lernmark, A., Nerup, J., & Richter-Olesen, H. (1980). Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ 51]CR-release. Medical Biology, 58(6), 322-328.

CBE

Kromann H, Christy M, Egeberg J, Lernmark A, Nerup J, Richter-Olesen H. 1980. Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ 51]CR-release. Medical Biology. 58(6):322-328.

MLA

Vancouver

Kromann H, Christy M, Egeberg J, Lernmark A, Nerup J, Richter-Olesen H. Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ 51]CR-release. Medical Biology. 1980 Dec 1;58(6):322-328.

Author

Kromann, H. ; Christy, M. ; Egeberg, J. ; Lernmark, A. ; Nerup, J. ; Richter-Olesen, H. / Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ 51]CR-release. In: Medical Biology. 1980 ; Vol. 58, No. 6. pp. 322-328.

RIS

TY - JOUR

T1 - Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ 51]CR-release

AU - Kromann, H.

AU - Christy, M.

AU - Egeberg, J.

AU - Lernmark, A.

AU - Nerup, J.

AU - Richter-Olesen, H.

PY - 1980/12/1

Y1 - 1980/12/1

N2 - Dispersed islet cells were prepared from collagenase-isolated lean mouse pancreatic islets by Dispase-II and subsequent mechanical treatment in calcium depleted media. An average yield of 600 cells per islet was obtained, 84% of the cells being β-cells. Cells were incubated with radioactive chromium as a marker of cell viability. Optimal labelling of 1-2 cpm per cell was obtained by incubating 10 5 cells with 10 6 cpm of [ 51]Cr for 90 min. When islet cells were incubated with streptozotocin, this drug induced [ 51]-Cr-release after a lag time of 2-4 hours. Furhermore, a positive correlation between streptozotocin concentrations and [ 51]Cr-release was found. This assay of cytotoxicity was highly reproducible and might be applicable in the study of other β-cell damaging agents or autoimmune phenomena in the pathogenesis of diabetes.

AB - Dispersed islet cells were prepared from collagenase-isolated lean mouse pancreatic islets by Dispase-II and subsequent mechanical treatment in calcium depleted media. An average yield of 600 cells per islet was obtained, 84% of the cells being β-cells. Cells were incubated with radioactive chromium as a marker of cell viability. Optimal labelling of 1-2 cpm per cell was obtained by incubating 10 5 cells with 10 6 cpm of [ 51]Cr for 90 min. When islet cells were incubated with streptozotocin, this drug induced [ 51]-Cr-release after a lag time of 2-4 hours. Furhermore, a positive correlation between streptozotocin concentrations and [ 51]Cr-release was found. This assay of cytotoxicity was highly reproducible and might be applicable in the study of other β-cell damaging agents or autoimmune phenomena in the pathogenesis of diabetes.

UR - http://www.scopus.com/inward/record.url?scp=0019271975&partnerID=8YFLogxK

M3 - Article

C2 - 6453261

AN - SCOPUS:0019271975

VL - 58

SP - 322

EP - 328

JO - Medical Biology

JF - Medical Biology

SN - 0302-2137

IS - 6

ER -