Dispersion of viable pig liver cells with collagenase

Research output: Contribution to journalArticle

Abstract

Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.

Details

Authors
Organisations
External organisations
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Other Clinical Medicine
  • Cancer and Oncology
  • Surgery
  • Pediatrics
  • Cell and Molecular Biology
Original languageEnglish
Pages (from-to)1219-1225
JournalLife Sciences
Volume17
Issue number8
Publication statusPublished - 1975
Publication categoryResearch
Peer-reviewedYes