Disposable polymeric high-density nanovial arrays for matrix assisted laser desorption/ionization-time of flight-mass spectrometry: I. Microstructure development and manufacturing

Research output: Contribution to journalArticle

Abstract

In order to meet the expected enormous demand for mass spectrometry (MS)through-, put as a result of the current efforts to completely map the human proteome, this paper presents a new concept for low-cost high-throughput protein identification by matrix assisted laser desorption/ionization-time of flight-(MALDI-TOF)-MS peptide mapping using disposable polymeric high-density nanovial MALDI target plates. By means of microfabrication technology precision engineered nanovial arrays are fabricated in polymer substrates such as polymethylmethacrylate (PMMA) and polycarbonate (PC). The target plate fabrication processes investigated were precision. micromilling, cold embossing and injection moulding (work in progress). Nanovial dimensions were 300, 400 or 500 mum. Typical array densities were 165 nanovials/cm(2), which corresponds to 3300 vials on a full Applied Biosystems MALDI target plate. Obtained MALDI data displayed equal mass resolution, accuracy, signal intensity for peptide standards as compared to high-density silicon nanovial arrays previously reported by our group [7], as well as conventional stainless steel or gold targets.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Engineering
  • Analytical Chemistry

Keywords

  • polymeric high-density matrix-assisted laser desorption, ionization, target plates, nanovial, microfabrication, peptides, array
Original languageEnglish
Pages (from-to)3978-3983
JournalElectrophoresis
Volume22
Issue number18
Publication statusPublished - 2001
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Biomedical Engineering (011200011)