Divergence of Hoxc8 early enhancer parallels diverged axial morphologies between mammals and fishes

Research output: Contribution to journalArticle


There is considerable interest in understanding how cis-regulatory modifications drive morphological changes across species. Because developmental regulatory genes, including Hox genes, are remarkably conserved, their noncoding regulatory regions are likely sources for variations. Modifications of Hox cis-regulatory elements have potential to alter Hox gene expression and, hence, axial morphologies. In vertebrates, differences in the axial levels of Hox gene expression correlate with differences in the number and relative position of thoracic vertebrae. Variation in cis-regulatory elements of Hox genes can be identified by comparative sequence and reporter gene analyses in transgenic mouse embryos. Using these approaches, we show a remarkable divergence of the Hoxc8 early enhancers between mammals and fishes representing diverse axial morphologies. Extensive restructuring of the Hoxc8 early enhancer including nucleotide substitutions, inversion, and divergence result in distinct patterns of reporter gene expression along the embryonic axis. Our results provide an evolutionary perspective on how the enhancer elements are engineered and support the hypothesis that remodeling of Hox regulatory elements in different species has played a significant role in generating morphological diversity.


  • S Anand
  • WCH Wang
  • DR Powell
  • SA Bolanowski
  • J Zhang
  • Christina Ledje
  • AB Pawashe
  • CT Amemiya
  • CS Shashikant
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Genetics
Original languageEnglish
Pages (from-to)15666-15669
JournalProceedings of the National Academy of Sciences
Issue number26
Publication statusPublished - 2003
Publication categoryResearch

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Genetics (Closed 2011) (011005100)