DNA barcoding of fish larvae reveals uncharacterised biodiversity in tropical peat swamps of New Guinea, Indonesia

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DNA barcoding of fish larvae reveals uncharacterised biodiversity in tropical peat swamps of New Guinea, Indonesia. / Wibowo, Arif; Wahlberg, Niklas; Vasemägi, Anti.

In: Marine and Freshwater Research, Vol. 68, No. 6, 2017, p. 1079-1087.

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TY - JOUR

T1 - DNA barcoding of fish larvae reveals uncharacterised biodiversity in tropical peat swamps of New Guinea, Indonesia

AU - Wibowo, Arif

AU - Wahlberg, Niklas

AU - Vasemägi, Anti

PY - 2017

Y1 - 2017

N2 - The Indonesian archipelago, Borneo, Sumatra and West New Guinea (Papua), hosts half of the world's known tropical peat swamps, which support a significant proportion of the estimated biodiversity on Earth. However, several species groups that inhabit peat swamp environments remain poorly characterised and their biology, particularly during early life stages, is not well understood. In the present study we characterised larval and juvenile fish biodiversity, as well as spatial and temporal variability, in a pristine peat swamp environment of the River Kumbe in West New Guinea, Indonesia, based on analysis of the mitochondrial cytochrome-c oxidase subunit 1 (COI) sequence (501bp). Altogether, 10 fish species were detected in the peat swamp habitat during the larval and juvenile stages, whereas 13 additional species were caught at older stages. Twelve species were detected only in a single site, whereas some species, such as the Western archerfish (Toxotes oligolepis) and Lorentz's grunter (Pingalla lorentzi), were observed in all sampling sites. The occurrence of fish larvae also varied temporally for several species. In contrast with many earlier DNA barcoding studies in fish, we were not able to determine the species identity for a large proportion of sequenced larvae (68%) because of the lack of corresponding COI sequences in the reference dataset. Unidentified sequences clustered into five separate monophyletic clades. Based on genetic divergences, the putative taxonomic origin for the five morphotypes are Atherinidae, Osteoglossidae, Terapontidae and Gobiidae.

AB - The Indonesian archipelago, Borneo, Sumatra and West New Guinea (Papua), hosts half of the world's known tropical peat swamps, which support a significant proportion of the estimated biodiversity on Earth. However, several species groups that inhabit peat swamp environments remain poorly characterised and their biology, particularly during early life stages, is not well understood. In the present study we characterised larval and juvenile fish biodiversity, as well as spatial and temporal variability, in a pristine peat swamp environment of the River Kumbe in West New Guinea, Indonesia, based on analysis of the mitochondrial cytochrome-c oxidase subunit 1 (COI) sequence (501bp). Altogether, 10 fish species were detected in the peat swamp habitat during the larval and juvenile stages, whereas 13 additional species were caught at older stages. Twelve species were detected only in a single site, whereas some species, such as the Western archerfish (Toxotes oligolepis) and Lorentz's grunter (Pingalla lorentzi), were observed in all sampling sites. The occurrence of fish larvae also varied temporally for several species. In contrast with many earlier DNA barcoding studies in fish, we were not able to determine the species identity for a large proportion of sequenced larvae (68%) because of the lack of corresponding COI sequences in the reference dataset. Unidentified sequences clustered into five separate monophyletic clades. Based on genetic divergences, the putative taxonomic origin for the five morphotypes are Atherinidae, Osteoglossidae, Terapontidae and Gobiidae.

UR - http://www.scopus.com/inward/record.url?scp=85020060894&partnerID=8YFLogxK

U2 - 10.1071/MF16078

DO - 10.1071/MF16078

M3 - Article

VL - 68

SP - 1079

EP - 1087

JO - Marine and Freshwater Research

T2 - Marine and Freshwater Research

JF - Marine and Freshwater Research

SN - 1448-6059

IS - 6

ER -