Dual transcriptome of the immediate neutrophil and Candida albicans interplay

Research output: Contribution to journalArticle

Abstract

Background: Neutrophils are traditionally considered transcriptionally inactive. Compared to other immune cells, little is known about their transcriptional profile during interaction with pathogens. Methods: We analyzed the meta-transcriptome of the neutrophil-Candida albicans interplay and the transcriptome of C. albicans challenged with neutrophil extracellular traps (NETs) by RNA-Seq, considering yeast and hypha individually in each approach. Results: The neutrophil response to C. albicans yeast and hyphae was dominated by a morphotype-independent core response. However, 11% of all differentially expressed genes were regulated in a specific manner when neutrophils encountered the hyphal form of C. albicans. While involving genes for transcriptional regulators, receptors, and cytokines, the neutrophil core response lacked typical antimicrobial effectors genes. Genes of the NOD-like receptor pathway, including NLRP3, were enriched. Neutrophil- and NET-provoked responses in C. albicans differed. At the same time, the Candida transcriptome upon neutrophil encounter and upon NET challenge included genes from various metabolic processes and indicate a mutual role of the regulators Tup1p, Efg1p, Hap43p, and Cap1p. Upon challenge with neutrophils and NETs, the overall Candida response was partially morphotype-specific. Yet again, actual oppositional regulation in yeasts and hyphae was only detected for the arginine metabolism in neutrophil-infecting C. albicans. Conclusions: Taken together, our study provides a comprehensive and quantitative transcript profile of the neutrophil-C. albicans interaction. By considering the two major appearances of both, neutrophils and C. albicans, our study reveals yet undescribed insights into this medically relevant encounter. Hence, our findings will facilitate future research and potentially inspire novel therapy developments.

Details

Authors
  • Maria J. Niemiec
  • Christian Grumaz
  • David Ermert
  • Christiane Desel
  • Madhu Shankar
  • José Pedro Lopes
  • Ian G. Mills
  • Philip Stevens
  • Kai Sohn
  • Constantin F. Urban
Organisations
External organisations
  • Umeå University
  • Leibniz Institute for Natural Product Research and Infection Biology
  • Fraunhofer Institute for Interfacial Engineering and Biotechnology
  • University Hospital Erlangen
  • University of Oxford
  • Queen's University Belfast
  • University of Stuttgart
  • University of Vienna
  • Friedrich-Alexander University Erlangen-Nürnberg
  • University of Oslo
  • Norwegian Radium Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area
  • Cell and Molecular Biology

Keywords

  • Candida, Dual transcriptome, Extracellular traps, Morphotype, Neutrophils, NOD-like receptor pathway, Nutritional immunity
Original languageEnglish
Article number696
JournalBMC Genomics
Volume18
Issue number1
Publication statusPublished - 2017 Sep 6
Publication categoryResearch
Peer-reviewedYes