Dynamic extraction coupled on-line to liquid chromatography with a parallel sampling interface—a proof of concept for monitoring extraction kinetics

Research output: Contribution to journalArticle


On-line hyphenation of extraction with chromatography has been explored in several different types of combinations. However, monitoring the complete process of a dynamic, continuous-flow extraction is not possible with any hyphenated system reported so far. The current work demonstrates that this challenging task can be effectively fulfilled by using a parallel sampling interface, which mimics the concept of comprehensive two-dimensional chromatography. In this study, pressurised hot water extraction was coupled on-line with ultra-high-performance liquid chromatography. The set-up was utilised in a kinetic study of dynamic pressurised hot water extraction of curcuminoids from turmeric powder. Compound-specific extraction curves were obtained, which clearly indicated the rate-limiting factors of the extraction processes under different conditions. Additionally, thermal degradation of curcumin during the extraction could also be demonstrated in some of the extractions.


Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry


  • Curcuminoids, Dynamic extraction, Liquid chromatography, On-line hyphenation, Parallel sampling, Turmeric powder
Original languageEnglish
Pages (from-to)3675-3683
JournalAnalytical and Bioanalytical Chemistry
Issue number16
Early online date2019 May 6
Publication statusPublished - 2019
Publication categoryResearch