Dynamic extraction coupled on-line to liquid chromatography with a parallel sampling interface—a proof of concept for monitoring extraction kinetics

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Abstract

On-line hyphenation of extraction with chromatography has been explored in several different types of combinations. However, monitoring the complete process of a dynamic, continuous-flow extraction is not possible with any hyphenated system reported so far. The current work demonstrates that this challenging task can be effectively fulfilled by using a parallel sampling interface, which mimics the concept of comprehensive two-dimensional chromatography. In this study, pressurised hot water extraction was coupled on-line with ultra-high-performance liquid chromatography. The set-up was utilised in a kinetic study of dynamic pressurised hot water extraction of curcuminoids from turmeric powder. Compound-specific extraction curves were obtained, which clearly indicated the rate-limiting factors of the extraction processes under different conditions. Additionally, thermal degradation of curcumin during the extraction could also be demonstrated in some of the extractions.

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Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry

Keywords

  • Curcuminoids, Dynamic extraction, Liquid chromatography, On-line hyphenation, Parallel sampling, Turmeric powder
Original languageEnglish
Pages (from-to)3675-3683
JournalAnalytical and Bioanalytical Chemistry
Volume411
Issue number16
Early online date2019 May 6
Publication statusPublished - 2019
Publication categoryResearch
Peer-reviewedYes