Dynamic perifusion to maintain and assess isolated pancreatic islets.

Research output: Contribution to journalArticle

Abstract

Advances in human islet transplant techniques are hampered by the inability to assess the quality of isolated islets. A flow culture system was developed to perifuse isolated pancreatic islets or cultured beta-cell lines in order to continuously and noninvasively assess cell function and viability with high kinetic resolution. Continuous perifusion of large amounts of islet tissue as isolated from human pancreata enables the use of noninvasive measurement technologies not previously applied to islets. To compare dynamic perifusion of tissue at high density with conventional static cultures, we measured glucose-stimulated insulin secretion and O2 consumption of large amounts of INS-1 cells (45-65 x 10(6)) to confirm that perifused cells were adequately supplied with oxygen and nutrients and remained functionally responsive. Isolated human and monkey islets that were perifused for 18 h showed robust biphasic insulin secretion in response to a step increase in glucose, demonstrating the a

Details

Authors
  • I R Sweet
  • D L Cook
  • R W Wiseman
  • C J Greenbaum
  • Åke Lernmark
  • S Matsumoto
  • J C Teague
  • K A Krohn
External organisations
  • External Organization - Unknown
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Endocrinology and Diabetes
Original languageEnglish
Pages (from-to)67-76
JournalDiabetes Technology & Therapeutics
Volume4
Issue number1
Publication statusPublished - 2002
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes