Dysregulation of CXCR3 expression on peripheral blood leukocytes in patients with neovascular age-related macular degeneration

Research output: Contribution to journalArticle


PURPOSE: The chemokine receptor CXCR3 has been strongly related to inhibition of angiogenesis. The purpose of this study was to investigate the association between expression of CXCR3 on peripheral blood leukocytes and age-related wet macular degeneration. Furthermore, we measured the plasma concentration of chemokines CXCL9 to -11.

METHODS: The study group consisted of patients with age-related macular degeneration (AMD) attending our department. Patients referred for reasons other than AMD were enrolled as control subjects. The expression of CXCR3 on T cells and the plasma concentration of CXCL9 to -11 were measured using flow cytometry.

RESULTS: We looked at all CD8(+) T cells expressing CXCR3 and found a significantly lower percentage of these cells in the neovascular AMD group compared to the age-matched control group (P = 0.05). When dividing the CD8(+) cells into functional groups according to their expression of CXCR3, we found a significantly lower percentage of CD8(+) CXCR3(high) cells in the group with neovascular AMD compared to the control group (P = 0.038). We found a lower percentage of CD4(+)CD69(+)CXCR3(+) T cells in the group of patients with neovascular AMD when compared to the age-matched control group (P = 0.052).

CONCLUSIONS: Our results point toward a systemic dysregulation of CXCR3 in patients with neovascular AMD. Since there is evidence to suggest that CXCR3 is able to alter the response of VEGF, the primary driver of choroidal neovascularization (CNV) formation, low levels of CXCR3 could potentially drive some patients toward a more angiogenic profile leading to CNV formation and growth. CXCR3-enhancing molecules could therefore be a possible target for treatment of AMD.


  • Mads Krüger Falk
  • Amardeep Singh
  • Carsten Faber
  • Mogens Holst Nissen
  • Thomas Hviid
  • Torben Lykke Sørensen
External organisations
  • University of Copenhagen
  • Copenhagen University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Ophthalmology


  • Aged, Biomarkers/blood, Disease Progression, Female, Flow Cytometry, Fluorescein Angiography, Fundus Oculi, Humans, Leukocytes/metabolism, Macular Degeneration/blood, Male, Receptors, CXCR3/biosynthesis, Retinal Neovascularization/blood, Tomography, Optical Coherence
Original languageEnglish
Pages (from-to)4050-4056
Number of pages7
JournalInvestigative Ophthalmology & Visual Science
Issue number7
Publication statusPublished - 2014 May 8
Publication categoryResearch
Externally publishedYes

Bibliographic note

Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.