Early evolution of the land plant circadian clock

Research output: Contribution to journalArticle

Abstract

While angiosperm clocks can be described as an intricate network of interlocked transcriptional feedback loops, clocks of green algae have been modelled as a loop of only two genes. To investigate the transition from a simple clock in algae to a complex one in angiosperms, we performed an inventory of circadian clock genes in bryophytes and charophytes. Additionally, we performed functional characterization of putative core clock genes in the liverwort Marchantia polymorpha and the hornwort Anthoceros agrestis. Phylogenetic construction was combined with studies of spatiotemporal expression patterns and analysis of M. polymorpha clock gene mutants. Homologues to core clock genes identified in Arabidopsis were found not only in bryophytes but also in charophytes, albeit in fewer copies. Circadian rhythms were detected for most identified genes in M. polymorpha and A. agrestis, and mutant analysis supports a role for putative clock genes in M. polymorpha. Our data are in line with a recent hypothesis that adaptation to terrestrial life occurred earlier than previously expected in the evolutionary history of charophyte algae. Both gene duplication and acquisition of new genes was important in the evolution of the plant circadian clock, but gene loss has also contributed to shaping the clock of bryophytes.

Details

Authors
  • Anna Malin Linde
  • D. Magnus Eklund
  • Akane Kubota
  • Eric R. A. Pederson
  • Karl Holm
  • Niclas Gyllenstrand
  • Ryuichi Nishihama
  • Nils Cronberg
  • Tomoaki Muranaka
  • Tokitaka Oyama
  • Takayuki Kohchi
  • Ulf Lagercrantz
Organisations
External organisations
  • Kyoto University
  • Linnean Centre for Plant Biology in Uppsala
  • Uppsala University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Botany
  • Evolutionary Biology

Keywords

  • Marchantia polymorpha, Bryophyte, Circadian clock, Evolution, Transcription factor
Original languageEnglish
Pages (from-to)576-590
JournalNew Phytologist
Volume216
Issue number2
Early online date2017 Feb 28
Publication statusPublished - 2017 Oct
Publication categoryResearch
Peer-reviewedYes