Effect of acute hypohydration on glycemic regulation in healthy adults: A randomized crossover trial

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Abstract

he aim of this study was to investigate the acute effect of hydration status on glycemic regulation in healthy adults and explore underlying mechanisms. In this randomized crossover trial, 16 healthy adults (8 men, 8 women) underwent an oral glucose tolerance test (OGTT) when hypohydrated and rehydrated after 4 days of pretrial standardization. One day before OGTT, participants were dehydrated for 1 h in a heat tent with subsequent fluid restriction (HYPO) or replacement (RE). The following day, an OGTT was performed with metabolic rate measurements and pre- and post-OGTT muscle biopsies. Peripheral quantitative computer tomography thigh scans were taken before and after intervention to infer changes in cell volume. HYPO (but not RE) induced 1.9% (SD 1.2) body mass loss, 2.9% (SD 2.7) cell volume reduction, and increased urinary hydration markers, serum osmolality, and plasma copeptin concentration (all P 0.007). Fasted serum glucose [HYPO 5.10 mmol/l (SD 0.42), RE 5.02 mmol/l (SD 0.40); P 0.327] and insulin [HYPO 27.1 pmol/l (SD 9.7), RE 27.6 pmol/l (SD 9.2); P 0.809] concentrations were similar between HYPO and RE. Hydration status did not alter the serum glucose (P 0.627) or insulin (P 0.200) responses during the OGTT. Muscle water content was lower before OGTT after HYPO compared with RE [761 g/kg wet wt (SD 13) vs. 772 g/kg wet wt (SD 18) RE] but similar after OGTT [HYPO 779 g/kg wet wt (SD 15) vs. RE 780 g/kg wet wt (SD 20); time P 0.011; trial time P 0.055]. Resting energy expenditure was similar between hydration states (stable between 1.21 and 5.94 kJ·kg 1 ·day 1 ; trial P 0.904). Overall, despite acute mild hypohydration increasing plasma copeptin concentrations and decreasing fasted cell volume and muscle water, we found no effect on glycemic regulation.

Details

Authors
  • Harriet A. Carroll
  • Iain Templeman
  • Yung Chih Chen
  • Robert M. Edinburgh
  • Elaine K. Burch
  • Jake T. Jewitt
  • Georgie Povey
  • Timothy D. Robinson
  • William L. Dooley
  • Robert Jones
  • Kostas Tsintzas
  • Widet Gallo
  • Olle Melander
  • Dylan Thompson
  • Lewis J. James
  • Laura Johnson
  • James A. Betts
Organisations
External organisations
  • University of Bath
  • University of Nottingham
  • Loughborough University
  • University of Bristol
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Physiology
Original languageEnglish
Pages (from-to)422-430
Number of pages9
JournalJournal of Applied Physiology
Volume126
Issue number2
Publication statusPublished - 2019
Publication categoryResearch
Peer-reviewedYes