Effect of Estramustine Phosphate on the Assembly of Isolated Bovine Brain Microtubules and Fast Axonal Transport in the Frog Sciatic Nerve

Research output: Contribution to journalArticle

Abstract

Estramustine phosphate (0.01 to 0.5 nriM), an estradiol mustard derivative used in the therapy of prostatic carcinoma, inhibited the assembly of brain microtubule proteins in vitro and disassembled preformed microtubules. In the presence of estramustine phosphate, the minimum microtubule-protein concentration sufficient for the assembly of microtubules was increased. Low concentrations of taxoi (20 μM) completely reversed the inhibition of assembly by estramustine phosphate. The effects were specific to estramustine phosphate since neither estradiol 170-phosphate, the hormonal moiety of the drug, nor nornitrogen mustard, the alkylating moiety, had any effect on assembly. Estramustine phosphate (0.1 to 0.5 HIM) was also found to reversibly inhibit fast axonal transport in the frog sciatic nerve. The nerve content of adenosine triphosphate, adenosine diphosphate, and adenosine monophosphate was not significantly affected by estramustine phosphate. Our results suggest that the cytotoxic action of estramustine phosphate could be dependent partially on an interaction with microtubules, probably via the microtubule-associated proteins.

Details

Authors
Organisations
External organisations
  • University of Gothenburg
  • AB Leo
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell Biology

Keywords

  • bovine brain microtubules, axonal transport
Original languageEnglish
Pages (from-to)2234-2239
Number of pages6
JournalCancer Research
Volume45
Issue number5
Publication statusPublished - 1985 May 1
Publication categoryResearch
Peer-reviewedYes