Effects of a topical enamel matrix derivative on skin wound healing

Research output: Contribution to journalArticle

Standard

Effects of a topical enamel matrix derivative on skin wound healing. / Mirastschijski, U; Konrad, D; Lundberg, E; Lyngstadaas, SP; Jorgensen, LN; Agren, MS.

In: Wound Repair and Regeneration, Vol. 12, No. 1, 2004, p. 100-108.

Research output: Contribution to journalArticle

Harvard

Mirastschijski, U, Konrad, D, Lundberg, E, Lyngstadaas, SP, Jorgensen, LN & Agren, MS 2004, 'Effects of a topical enamel matrix derivative on skin wound healing', Wound Repair and Regeneration, vol. 12, no. 1, pp. 100-108.

APA

Mirastschijski, U., Konrad, D., Lundberg, E., Lyngstadaas, SP., Jorgensen, LN., & Agren, MS. (2004). Effects of a topical enamel matrix derivative on skin wound healing. Wound Repair and Regeneration, 12(1), 100-108.

CBE

Mirastschijski U, Konrad D, Lundberg E, Lyngstadaas SP, Jorgensen LN, Agren MS. 2004. Effects of a topical enamel matrix derivative on skin wound healing. Wound Repair and Regeneration. 12(1):100-108.

MLA

Vancouver

Mirastschijski U, Konrad D, Lundberg E, Lyngstadaas SP, Jorgensen LN, Agren MS. Effects of a topical enamel matrix derivative on skin wound healing. Wound Repair and Regeneration. 2004;12(1):100-108.

Author

Mirastschijski, U ; Konrad, D ; Lundberg, E ; Lyngstadaas, SP ; Jorgensen, LN ; Agren, MS. / Effects of a topical enamel matrix derivative on skin wound healing. In: Wound Repair and Regeneration. 2004 ; Vol. 12, No. 1. pp. 100-108.

RIS

TY - JOUR

T1 - Effects of a topical enamel matrix derivative on skin wound healing

AU - Mirastschijski, U

AU - Konrad, D

AU - Lundberg, E

AU - Lyngstadaas, SP

AU - Jorgensen, LN

AU - Agren, MS

PY - 2004

Y1 - 2004

N2 - Enamel matrix derivative, obtained from developing porcine teeth, is composed mainly of amelogenin proteins and used topically in periodontal surgery for advanced periodontitis to regenerate lost connective tissues. The primary objective of this study was to investigate the effects of enamel matrix derivative on skin wound healing. Secondly, in vitro effects of enamel matrix derivative on dermal fibroblasts and microvascular endothelial cells were examined. Full-thickness, circular 2-cm skin wounds in white 16-week-old rabbits were treated thrice weekly with enamel matrix derivative (30 mg/ml) in the vehicle propylene glycol alginate or with vehicle alone. Enamel matrix derivative treatment increased the amount of granulation tissue and accelerated time to complete epithelialization by 3 days (p < 0.001) compared to vehicle treatment. In cultured fibroblasts, vascular endothelial growth factor levels in conditioned media were increased more than fivefold (p < 0.001) with enamel matrix derivative treatment (0.1 mg/ml) over control, measured by specific enzyme-linked immunosorbent assay. Enamel matrix derivative also increased release of matrix metalloproteinase-2 more than threefold from fibroblasts (p < 0.001) and from endothelial cells (p < 0.001). Thus, enamel matrix derivative significantly accelerated wound closure in rabbits, possibly by increasing levels of growth factors and proteinases important for granulation tissue formation and remodeling.

AB - Enamel matrix derivative, obtained from developing porcine teeth, is composed mainly of amelogenin proteins and used topically in periodontal surgery for advanced periodontitis to regenerate lost connective tissues. The primary objective of this study was to investigate the effects of enamel matrix derivative on skin wound healing. Secondly, in vitro effects of enamel matrix derivative on dermal fibroblasts and microvascular endothelial cells were examined. Full-thickness, circular 2-cm skin wounds in white 16-week-old rabbits were treated thrice weekly with enamel matrix derivative (30 mg/ml) in the vehicle propylene glycol alginate or with vehicle alone. Enamel matrix derivative treatment increased the amount of granulation tissue and accelerated time to complete epithelialization by 3 days (p < 0.001) compared to vehicle treatment. In cultured fibroblasts, vascular endothelial growth factor levels in conditioned media were increased more than fivefold (p < 0.001) with enamel matrix derivative treatment (0.1 mg/ml) over control, measured by specific enzyme-linked immunosorbent assay. Enamel matrix derivative also increased release of matrix metalloproteinase-2 more than threefold from fibroblasts (p < 0.001) and from endothelial cells (p < 0.001). Thus, enamel matrix derivative significantly accelerated wound closure in rabbits, possibly by increasing levels of growth factors and proteinases important for granulation tissue formation and remodeling.

M3 - Article

VL - 12

SP - 100

EP - 108

JO - Wound Repair and Regeneration

T2 - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1524-475X

IS - 1

ER -