Effects of clodronate on cortical and trabecular bone in ovariectomized rats on a low calcium diet

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Effects of clodronate on cortical and trabecular bone in ovariectomized rats on a low calcium diet. / Kaastad, T S; Reikeras, O; Madsen, J E; Narum, S; Stromme, J H; Obrant, Karl; Nordsletten, L.

In: Calcified Tissue International, Vol. 61, No. 2, 1997, p. 158-164.

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Harvard

Kaastad, TS, Reikeras, O, Madsen, JE, Narum, S, Stromme, JH, Obrant, K & Nordsletten, L 1997, 'Effects of clodronate on cortical and trabecular bone in ovariectomized rats on a low calcium diet', Calcified Tissue International, vol. 61, no. 2, pp. 158-164. https://doi.org/10.1007/s002239900315

APA

Kaastad, T. S., Reikeras, O., Madsen, J. E., Narum, S., Stromme, J. H., Obrant, K., & Nordsletten, L. (1997). Effects of clodronate on cortical and trabecular bone in ovariectomized rats on a low calcium diet. Calcified Tissue International, 61(2), 158-164. https://doi.org/10.1007/s002239900315

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Kaastad, T S ; Reikeras, O ; Madsen, J E ; Narum, S ; Stromme, J H ; Obrant, Karl ; Nordsletten, L. / Effects of clodronate on cortical and trabecular bone in ovariectomized rats on a low calcium diet. In: Calcified Tissue International. 1997 ; Vol. 61, No. 2. pp. 158-164.

RIS

TY - JOUR

T1 - Effects of clodronate on cortical and trabecular bone in ovariectomized rats on a low calcium diet

AU - Kaastad, T S

AU - Reikeras, O

AU - Madsen, J E

AU - Narum, S

AU - Stromme, J H

AU - Obrant, Karl

AU - Nordsletten, L

PY - 1997

Y1 - 1997

N2 - The aim of this study was to evaluate the contribution of a low calcium diet to the cortical and trabecular osteoporosis seen in ovariectomized rats after 7 weeks on a low calcium diet and to investigate the effects of the bisphosphonate clodronate on this development of osteoporosis. Thirty-six mature, female Wistar rats were randomized into four groups: Ovx-B (bisphosphonate) and Ovx-C (control) were ovariectomized, and Sham-Ca (low calcium) and Sham+Ca (normal calcium) were sham operated. The first three groups were fed a low calcium diet (0.01%) and Sham+Ca normal rat chow (Ca 1.1%). The Ovx-B received 10 mg/kg s.c. clodronate daily for nine weeks, and Ovx-C, Sham-Ca, and Sham+Ca received the same volumes of saline. Bone mineral turnover measured as 85Sr-uptake was increased in all low calcium groups compared to Sham+Ca. The Sham+Ca femora had higher dry weight and ash weight than the other groups, and Ovx-C had higher dry weight compared with Ovx-B and Sham-Ca. Calcium content was lower in both Ovx groups compared to both Sham groups. Magnesium was lower in all groups compared to Sham+Ca and higher in Ovx-B compared with Ovx-C. In the femoral shaft, Sham+Ca had significantly higher ultimate bending moment, energy absorption, and deflection compared to the other three groups. Ultimate bending moment was higher in Sham-Ca than in Ovx-C. Stiffness was increased in both Sham+Ca and Ovx-B compared to Ovx-C. The maximum stress in the femoral midshaft was higher in Sham+Ca than in the other groups, and higher in Ovx-B than in Ovx-C. Histomorphometry showed increased medullary area in all low calcium groups compared to Sham+Ca and larger cortical area in Sham+Ca and Ovx-B compared to Ovx-C. Compared to Sham+Ca the trabecular bone volume was decreased to 30% in Sham-Ca and to 9% in Ovx-C, but was unchanged in Ovx-B. The low calcium diet generally increased bone mineral turnover and reduced the tibial bone volume. Femoral changes led to a reduction of cortical fracture strength and maximal stress. Ovariectomy in addition to a low calcium diet reduced femoral strength even more. Daily injections of clodronate to ovariectomized rats on a low calcium diet increased femoral shaft stiffness and maximum stress, and clodronate preserved both trabecular and cortical tibial bone volume completely.

AB - The aim of this study was to evaluate the contribution of a low calcium diet to the cortical and trabecular osteoporosis seen in ovariectomized rats after 7 weeks on a low calcium diet and to investigate the effects of the bisphosphonate clodronate on this development of osteoporosis. Thirty-six mature, female Wistar rats were randomized into four groups: Ovx-B (bisphosphonate) and Ovx-C (control) were ovariectomized, and Sham-Ca (low calcium) and Sham+Ca (normal calcium) were sham operated. The first three groups were fed a low calcium diet (0.01%) and Sham+Ca normal rat chow (Ca 1.1%). The Ovx-B received 10 mg/kg s.c. clodronate daily for nine weeks, and Ovx-C, Sham-Ca, and Sham+Ca received the same volumes of saline. Bone mineral turnover measured as 85Sr-uptake was increased in all low calcium groups compared to Sham+Ca. The Sham+Ca femora had higher dry weight and ash weight than the other groups, and Ovx-C had higher dry weight compared with Ovx-B and Sham-Ca. Calcium content was lower in both Ovx groups compared to both Sham groups. Magnesium was lower in all groups compared to Sham+Ca and higher in Ovx-B compared with Ovx-C. In the femoral shaft, Sham+Ca had significantly higher ultimate bending moment, energy absorption, and deflection compared to the other three groups. Ultimate bending moment was higher in Sham-Ca than in Ovx-C. Stiffness was increased in both Sham+Ca and Ovx-B compared to Ovx-C. The maximum stress in the femoral midshaft was higher in Sham+Ca than in the other groups, and higher in Ovx-B than in Ovx-C. Histomorphometry showed increased medullary area in all low calcium groups compared to Sham+Ca and larger cortical area in Sham+Ca and Ovx-B compared to Ovx-C. Compared to Sham+Ca the trabecular bone volume was decreased to 30% in Sham-Ca and to 9% in Ovx-C, but was unchanged in Ovx-B. The low calcium diet generally increased bone mineral turnover and reduced the tibial bone volume. Femoral changes led to a reduction of cortical fracture strength and maximal stress. Ovariectomy in addition to a low calcium diet reduced femoral strength even more. Daily injections of clodronate to ovariectomized rats on a low calcium diet increased femoral shaft stiffness and maximum stress, and clodronate preserved both trabecular and cortical tibial bone volume completely.

KW - Ovariectomy

KW - Low calcium diet

KW - Clodronate

KW - Bone volume

KW - Bone strength

U2 - 10.1007/s002239900315

DO - 10.1007/s002239900315

M3 - Article

VL - 61

SP - 158

EP - 164

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 1432-0827

IS - 2

ER -