Effects of dextran-linked chloromercuribenzoic acid on insulin release from microdissected pancreatic islets

Research output: Contribution to journalArticle

Abstract

Insulin release in response to dextran-linked p-chloromercuribenzoic acid was studied in microdissected pancreatic islets of non-inbred ob/ob-mice. No contamination of the dextran-linked mercurial with free chloromercuribenzoic acid was detected before or after the incubation with islets. In comparison with free mercurial, of the same thiol-blocking activity, the dextran-linked compound had a weak insulin-releasing action with a different dose vs. response relationship. The dextran-linked mercurial had no demonstrable effect on the islet content of cyclic AMP. The results support the hypothesis that free organic mercurials mainly stimulate insulin release by blocking thiol groups that are embedded within the β-cell plasma membranes beneath their surfaces.

Details

Authors
  • Stig Åkerstrom
  • Bo Hellman
  • Åker Lernmark
  • Bernt Lindberg
  • Monica Söderberg
  • Inge Bert Täljedal
External organisations
  • Umeå University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Endocrinology and Diabetes
Original languageEnglish
Pages (from-to)96-105
Number of pages10
JournalBBA - General Subjects
Volume451
Issue number1
Publication statusPublished - 1976 Nov 18
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes