Effects of ionic substances in bleaching filtrates and of lignosulfonates on the activity of oxalate oxidase from barley

Research output: Contribution to journalArticle


The effects of ionic substances in seven industrial filtrates from kraft pulping, mechanical pulping, and sulfite pulping on the activity of oxalate oxidase from barley were investigated by pre-treatment of the filtrates with ion-exchange resins prior to enzymatic degradation of the oxalic acid in the filtrates. The pre-treatment resulted in increased oxalic acid degradation rates in all filtrates, except for one that was obtained from sulfite pulping. The possibility that lignosulfonates, which were present in the filtrate from sulfite pulping, could affect oxalate oxidase was investigated in a separate set of experiments involving four different preparations of lignosulfonates. At a lignosulfonate concentration of 50 mg/mL and a pH of 3.8, only 2-16% of the activity of oxalate oxidase remained. The results show the effects of anionic and cationic substances in bleaching filtrates on oxalate oxidase and indicate that there is an interaction between the enzyme, which has a positive net charge at pH 3.8, and the polymeric anionic lignosulfonates.


  • Sandra Winestrand
  • Simona Larsson
  • Pierre Cassland
  • Nils-Olof Nilvebrant
  • Leif J. Jonsson
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology


  • Lignosulfonates, Oxalate oxidase, Pulp and paper
Original languageEnglish
Pages (from-to)245-252
JournalEngineering in Life Sciences
Issue number3
Publication statusPublished - 2011
Publication categoryResearch