Effects of pentobarbital on GABA-activated currents in acutely-isolated rat dentate gyrus granule neurons

Research output: Contribution to journalArticle

Abstract

Granule neurons from the rat dentate gyrus were acutely isolated and whole-cell currents recorded. Maximal enhancement of 7 muM gamma-aminobutyric acid (GABA; EC30) evoked currents was obtained with 100 muM pentobarbital where the peak-current was 2.1 +/- 0.2 of control. One hundred muM pentobarbital alone evoked no current response whereas I mM pentobarbital elicited a current response that was 0.4 +/- 0.2 of the 100 muM GABA-activated peak current. In 100 muM pentobarbital, the GABA EC50 value shifted from 14 to 3 muM but the peak-saturating-current value was not altered. An off-current was recorded on removal of 100 muM and higher pentobarbital concentrations. Ten mM pentobarbital abolished the peak-current response to 7 and 100 muM GABA. The results show that in the granule neurons the drug potency differs for the different effects of pentobarbital at GABA(A) receptors with the modulatory and inhibitory effects requiring lower concentrations than the direct activation of the receptors. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

Details

Authors
  • MSF Lim
  • Catarina Lindquist
  • Bryndis Birnir
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences

Keywords

  • receptor, inhibition, barbiturates, gamma-aminobutyric acid(A), hippocampus, ion channel
Original languageEnglish
Pages (from-to)139-142
JournalNeuroscience Letters
Volume353
Issue number2
Publication statusPublished - 2003
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: GABA Channels in Physiology and Pharmacology (013241570)