Effects of substrate concentration on methane potential and degradation kinetics in batch anaerobic digestion

Research output: Contribution to journalArticle


In this study, two experiments were conducted to evaluate the impact of substrate concentrations on methane potential and degradation kinetics of substrate. The biochemical methane potential (BMP) tests in Experiment I were performed at a constant inoculum to substrate ratio (ISR), whereas, different ISRs were applied in Experiment II. Results obtained from Experiment I revealed that methane potential of substrate increased at a saturating trend with higher substrate concentrations, and could differ by up to 30% between the lowest and highest investigated concentrations. The results of Experiment II verified the results of Experiment I, and further showed that this trend also occurs when the substrate concentration is regulated with ISRs. In contrast, substrate concentration had no significant impact on the degradation kinetics. It was concluded that dilutions should be avoided when the substrate concentration is lower than 10 g VS/L in order to avoid underestimations of methane potential from BMP test. (C) 2015 Elsevier Ltd. All rights reserved.


  • Bing Wang
  • Sten Strömberg
  • Chao Li
  • Nges Ivo Achu
  • Mihaela Nistor
  • Liangwei Deng
  • Jing Liu
External organisations
  • Bioprocess Control AB
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology


  • Anaerobic digestion, Biochemical methane potential, Substrate load, Substrate concentration, Kinetics
Original languageEnglish
Pages (from-to)240-246
JournalBioresource Technology
Publication statusPublished - 2015
Publication categoryResearch