Efficient poly(3-hydroxypropionate) production from glycerol using Lactobacillus reuteri and recombinant Escherichia coli harboring L. reuteri propionaldehyde dehydrogenase and Chromobacterium sp. PHA synthase genes.

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Bibtex

@article{da8e7d195b6c4ec78b9f6e674da6e156,
title = "Efficient poly(3-hydroxypropionate) production from glycerol using Lactobacillus reuteri and recombinant Escherichia coli harboring L. reuteri propionaldehyde dehydrogenase and Chromobacterium sp. PHA synthase genes.",
abstract = "Poly(3-hydroxypropionate), P(3HP), is a polymer combining good biodegradability with favorable material properties. In the present study, a production system for P(3HP) was designed, comprising conversion of glycerol to 3-hydroxypropionaldehyde (3HPA) as equilibrium mixture with 3HPA-hydrate and -dimer in aqueous system (reuterin) using resting cells of native Lactobacillus reuteri in a first stage followed by transformation of the 3HPA to P(3HP) using recombinant Escherichia coli strain co-expressing highly active coenzyme A-acylating propionaldehyde dehydrogenase (PduP) from L. reuteri and polyhydroxyalkanoate synthase (PhaCcs) from Chromobacterium sp. P(3HP) content of up to 40{\%} (w/w) cell dry weight was reached, and the yield with respect to the reuterin consumed by the cells was 78{\%}. Short biotransformation period (4.5h), lack of additives or expensive cofactors, and use of a cheap medium for cultivation of the recombinant strain, provides a new efficient and potentially economical system for P(3HP) production.",
author = "Javier Linares-Past{\'e}n and {Sabet Azad}, Ramin and Laura Pessina and Sardari, {Roya R R} and Ibrahim, {Mohammad H A} and Rajni Hatti-Kaul",
year = "2015",
doi = "10.1016/j.biortech.2014.12.099",
language = "English",
volume = "180",
pages = "172--176",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier",

}