Efficient sample preparation in immuno-matrix-assisted laser desorption/ionization mass spectrometry using acoustic trapping

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Abstract

Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectrometry using only half a million 2.8 mu m antibody covered beads is presented. The acoustic trapping is done in 200 x 2000 mu m2 glass capillaries and provides highly efficient binding and washing conditions, as shown by complete removal of detergents and sample processing times of 5-10 min. The versatility of the method is demonstrated using an antibody against Angiotensin I (Ang I), a peptide hormone involved in hypotension. Using this model system, the acoustic trapping was efficient in enriching Angiotensin at 400 pM spiked in plasma samples. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4798473]

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Engineering

Keywords

  • acoustic applications, biological specimen preparation, molecular, biophysics, organic compounds, time of flight mass spectroscopy
Original languageEnglish
Article number024107
JournalBiomicrofluidics
Volume7
Issue number2
Publication statusPublished - 2013
Publication categoryResearch
Peer-reviewedYes