Efficient sample preparation in immuno-matrix-assisted laser desorption/ionization mass spectrometry using acoustic trapping

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Bibtex

@article{c8935c4c35bc49739d66e185a440c974,
title = "Efficient sample preparation in immuno-matrix-assisted laser desorption/ionization mass spectrometry using acoustic trapping",
abstract = "Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectrometry using only half a million 2.8 mu m antibody covered beads is presented. The acoustic trapping is done in 200 x 2000 mu m2 glass capillaries and provides highly efficient binding and washing conditions, as shown by complete removal of detergents and sample processing times of 5-10 min. The versatility of the method is demonstrated using an antibody against Angiotensin I (Ang I), a peptide hormone involved in hypotension. Using this model system, the acoustic trapping was efficient in enriching Angiotensin at 400 pM spiked in plasma samples. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4798473]",
keywords = "acoustic applications, biological specimen preparation, molecular, biophysics, organic compounds, time of flight mass spectroscopy",
author = "Bj{\"o}rn Hammarstr{\"o}m and Hong Yan and Johan Nilsson and Simon Ekstr{\"o}m",
year = "2013",
doi = "10.1063/1.4798473",
language = "English",
volume = "7",
journal = "Biomicrofluidics",
issn = "1932-1058",
publisher = "American Institute of Physics",
number = "2",

}