Electrical wiring of Pseudomonas putida and Pseudomonas fluorescens with osmium redox polymers

Research output: Contribution to journalArticle


Two different flexible osmium redox polymers; poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-di'pyridyl)(2)Cl-2](2 +/+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)(3)](2+/3+) (osmium redox polymer II) were investigated for their ability to efficiently "wire" Pseudomonas putida ATCC 126633 and Pseudomonas fluorescens (P putida DSM 6521), which are well-known phenol degrading organisms, when entrapped onto cysteamine modified gold electrodes. The two Os-polymers differ in redox potential and the length of the side chains, where the OS2+/3+-functionalities are located. The bacterial cells were adapted to grow in the presence of phenol as the sole source of organic carbon. The performance of the redox polymers as mediators was investigated for making microbial sensors. The analytical characteristics of the microbial sensors were evaluated for determination of catechol, phenol and glucose as substrates in both batch analysis and flow analysis mode.


  • Suna Timur
  • Behzad Haghighi
  • Jan Tkac
  • Nurdan Pazarhoglu
  • Azmi Telefoncu
  • Lo Gorton
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry


  • osmium redox polymers, microbial biosensor
Original languageEnglish
Pages (from-to)38-45
Issue number1
Publication statusPublished - 2007
Publication categoryResearch

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)