Electrochemically induced oxidative damage to double-stranded calf thymus DNA adsorbed on gold electrodes

Research output: Contribution to journalArticle

Abstract

Electrochemically induced oxidative damage to DNA was studied with double-stranded calf thymus DNA immobilized directly on a gold electrode surface. Pre-polarization of the DNA-modified electrodes at +0.5 V versus Ag/AgCl reference electrode, in a free from DNA blank buffer solution, pH 7.4, allowed for subsequent detection of direct electrochemical oxidation of adsorbed on gold DNA, in the potential range from +0.7 to +0.8 V. The redox potential of the process corresponded to the potentials of the oxidation of guanine bases in DNA. It is shown that with increasing potential scan rate, v, the mechanism of electrochemical oxidation of DNA changes from the irreversible 4e(-) oxidative damage of DNA at low v to reversible 1e(-) oxidation at high nu, keeping the electrochemical activity of the adsorbed DNA layer virtually the same.

Details

Authors
  • Elena Ferapontova
  • SV Shipovskov
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry

Keywords

  • gold electrode, oxidative DNA damage, guanine
Original languageEnglish
Pages (from-to)99-104
JournalBiochemistry
Volume68
Issue number1
Publication statusPublished - 2003
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)