Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome.

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Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome. / Gu Urban, Gucci Jijuan; Friedman, Mikaela; Ren, Ping; Törn, Carina; Fex, Malin; Hampe, Christiane S; Lernmark, Åke; Landegren, Ulf; Kamali-Moghaddam, Masood.

In: Scientific Reports, Vol. 5, 11196, 2015.

Research output: Contribution to journalArticle

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Gu Urban, GJ, Friedman, M, Ren, P, Törn, C, Fex, M, Hampe, CS, Lernmark, Å, Landegren, U & Kamali-Moghaddam, M 2015, 'Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome.', Scientific Reports, vol. 5, 11196. https://doi.org/10.1038/srep11196

APA

Gu Urban, G. J., Friedman, M., Ren, P., Törn, C., Fex, M., Hampe, C. S., Lernmark, Å., Landegren, U., & Kamali-Moghaddam, M. (2015). Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome. Scientific Reports, 5, [11196]. https://doi.org/10.1038/srep11196

CBE

Gu Urban GJ, Friedman M, Ren P, Törn C, Fex M, Hampe CS, Lernmark Å, Landegren U, Kamali-Moghaddam M. 2015. Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome. Scientific Reports. 5:Article 11196. https://doi.org/10.1038/srep11196

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Author

Gu Urban, Gucci Jijuan ; Friedman, Mikaela ; Ren, Ping ; Törn, Carina ; Fex, Malin ; Hampe, Christiane S ; Lernmark, Åke ; Landegren, Ulf ; Kamali-Moghaddam, Masood. / Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome. In: Scientific Reports. 2015 ; Vol. 5.

RIS

TY - JOUR

T1 - Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome.

AU - Gu Urban, Gucci Jijuan

AU - Friedman, Mikaela

AU - Ren, Ping

AU - Törn, Carina

AU - Fex, Malin

AU - Hampe, Christiane S

AU - Lernmark, Åke

AU - Landegren, Ulf

AU - Kamali-Moghaddam, Masood

PY - 2015

Y1 - 2015

N2 - Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence, and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and GAD65-GADA levels were significantly higher in serum samples from SPS patients compared to healthy controls. The proximity ligation assays applied for detection of GAD65 and its immune complexes may thus enable improved diagnosis and better understanding of SPS.

AB - Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence, and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and GAD65-GADA levels were significantly higher in serum samples from SPS patients compared to healthy controls. The proximity ligation assays applied for detection of GAD65 and its immune complexes may thus enable improved diagnosis and better understanding of SPS.

U2 - 10.1038/srep11196

DO - 10.1038/srep11196

M3 - Article

C2 - 26080009

VL - 5

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 11196

ER -