Engineered xyloglucan specificity in a carbohydrate-binding module.

Research output: Contribution to journalArticle

Abstract

The field of plant cell wall biology is constantly growing and consequently so is the need for more sensitive and specific probes for individual wall components. Xyloglucan is a key polysaccharide widely distributed in the plant kingdom in both structural and storage tissues that exist in both fucosylated and non-fucosylated variants. Presently, the only xyloglucan marker available is the monoclonal antibody CCRC-M1 that is specific to terminal -1,2-linked fucosyl residues on xyloglucan oligo- and polysaccharides. As a viable alternative to searches for natural binding proteins or creation of new monoclonal antibodies, an approach to select xyloglucan-specific binding proteins from a combinatorial library of the carbohydrate-binding module, CBM4-2, from xylanase Xyn10A of Rhodothermus marinus is described. Using phage display technology in combination with a chemoenzymatic method to anchor xyloglucan to solid supports, the selection of xyloglucan-binding modules with no detectable residual wild-type xylan and ß-glucan-binding ability was achieved.

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Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • binding specificity, molecular engineering, xyloglucan, phage display, carbohydrate-binding module
Original languageEnglish
Pages (from-to)1171-1180
JournalGlycobiology
Volume16
Issue number12
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes

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