Enzyme conformational alterations detected by partition column chromatography

Research output: Contribution to journalArticle

Abstract

In this paper, we demonstrate the ability of liquid-liquid partition chromatography (LLPC) to detect conformational alterations occurring in well-characterized enzymes. The conformational changes induced in dehydrogenases such as alcohol dehydrogenase (ADH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenases (LDH) and malate dehydrogenase (MDH) upon binding of ligand(s) were detectable by LLPC. The ligand-dependent equilibrium between two forms of citrate synthase (CS), glutamate-oxaloacetate transaminase (GOT), hexokinase (HK) and 3-phosphoglycerate kinase (PGK) could also be demonstrated. Furthermore, different conformational forms of some of the apoenzymes could also be detected and separated by LLPC. The results obtained here are discussed in relation to those obtained by other methods.

Details

Authors
  • Christer Wingren
  • Ulla-Britt Hansson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • Partition column chromatography, Conformational changes, Enzymes
Original languageEnglish
Pages (from-to)113-122
JournalJournal of Chromatography. B
Volume680
Issue number1-2
Publication statusPublished - 1996
Publication categoryResearch
Peer-reviewedYes