Estrogen upregulates hepatic apolipoprotein M expression via the estrogen receptor

Research output: Contribution to journalArticle

Abstract

Apolipoprotein M (apoM) is present predominantly in high-density lipoprotein (HDL) in human plasma, thus possibly involved in the regulation of HDL metabolism and the process of atherosclerosis. Although estrogen replacement therapy increases serum levels of apoAl and HDL, it does not seem to reduce the cardiovascular risk in postmenopausal women. Therefore, we investigated the effects of estrogen on apoM expression in vitro and in vivo. HepG2 cells were incubated with different concentrations of estrogen with or without the estrogen receptor antagonist, fulvestrant, and apoM expression in the cells was determined. Hepatic apoM expression and serum levels of apoM were also determined in normal and in ovariectomized rats treated with either placebo or estradiol benzoate, using sham operated rats as controls. Estrogen significantly increased mRNA levels of apoM and apoAl in HepG2 cell cultures in a dose- and time-dependent manner; the upregulation of both apolipoproteins was fully abolished by addition of estrogen receptor antagonist In normal rats, estrogen treatment led to an increase in plasma lipid levels including HDL cholesterol, a marked upregulation of apoM mRNA and a significant increase in serum levels of apoM. The same pattern of regulation was found in ovariectomized rats treated with estrogen. Thus, estrogen upregulates apoM expression both in vivo and in vitro by mechanism(s) involving the estrogen receptor. (C) 2011 Elsevier B.V. All rights reserved.

Details

Authors
  • Jiang Wei
  • Yuanping Shi
  • Xiaoying Zhang
  • Yuehua Feng
  • Guanghua Luo
  • Jun Zhang
  • Qinfeng Mu
  • Yanhong Tang
  • Yang Yu
  • Lili Pan
  • Peter Nilsson-Ehle
  • Ning Xu
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry
  • Pharmacology and Toxicology

Keywords

  • Apolipoprotein M, HDL, Estrogen, Lipid metabolism
Original languageEnglish
Pages (from-to)1146-1151
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1811
Issue number12
Publication statusPublished - 2011
Publication categoryResearch
Peer-reviewedYes

Total downloads

No data available