Ethanol effects on bradykinin-stimulated phosphoinositide hydrolysis in NG 108-15 neuroblastoma-glioma cells

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Ethanol effects on bradykinin-stimulated phosphoinositide hydrolysis in NG 108-15 neuroblastoma-glioma cells. / Simonsson, Per; Sun, Grace Y; Vecsei, Laszlo; Alling, Christer.

In: Alcohol, Vol. 6, No. 6, 1989, p. 475-479.

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Simonsson, Per ; Sun, Grace Y ; Vecsei, Laszlo ; Alling, Christer. / Ethanol effects on bradykinin-stimulated phosphoinositide hydrolysis in NG 108-15 neuroblastoma-glioma cells. In: Alcohol. 1989 ; Vol. 6, No. 6. pp. 475-479.

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TY - JOUR

T1 - Ethanol effects on bradykinin-stimulated phosphoinositide hydrolysis in NG 108-15 neuroblastoma-glioma cells

AU - Simonsson, Per

AU - Sun, Grace Y

AU - Vecsei, Laszlo

AU - Alling, Christer

PY - 1989

Y1 - 1989

N2 - The effect of short-and long-term ethanol exposure on bradykinin-stimulated hydrolysis of phosphatidylinositol 4.5-bisphosphate (PIP2) was investigated in neuroblastoma X glioma hybrid cells (NG 108-15). Acute exposure of 50-150 mM ethanol neither influenced the bradykinin-stimulated accumulation of [3H]-inositol phosphates (IP1, IP2, IP3) nor the hydrolysis of PIP2 in cells labelled with [3H]-inositol. Furthermore, ethanol (100 mM) added in the absence of agonist did not influence these parameters. However, in cells cultivated for 4 days in 100 mM ethanol, PIP2 hydrolysis and IP1, IP2 and IP3 formation after stimulation by 10(-6)-10(-5) M bradykinin was markedly inhibited while there was no effect on the basal levels or on the levels found after stimulation with low concentrations of bradykinin. The inhibitory effect of ethanol on IP accumulation became significant after 2-3 days of ethanol.

AB - The effect of short-and long-term ethanol exposure on bradykinin-stimulated hydrolysis of phosphatidylinositol 4.5-bisphosphate (PIP2) was investigated in neuroblastoma X glioma hybrid cells (NG 108-15). Acute exposure of 50-150 mM ethanol neither influenced the bradykinin-stimulated accumulation of [3H]-inositol phosphates (IP1, IP2, IP3) nor the hydrolysis of PIP2 in cells labelled with [3H]-inositol. Furthermore, ethanol (100 mM) added in the absence of agonist did not influence these parameters. However, in cells cultivated for 4 days in 100 mM ethanol, PIP2 hydrolysis and IP1, IP2 and IP3 formation after stimulation by 10(-6)-10(-5) M bradykinin was markedly inhibited while there was no effect on the basal levels or on the levels found after stimulation with low concentrations of bradykinin. The inhibitory effect of ethanol on IP accumulation became significant after 2-3 days of ethanol.

KW - Phosphatidylinositol 4

KW - 5-bisphosphate

KW - Inositol phosphates

KW - Phospholipase C

KW - NG 108-15 cell-line

KW - Bradykinin

KW - Ethanol

U2 - 10.1016/0741-8329(89)90055-4

DO - 10.1016/0741-8329(89)90055-4

M3 - Article

VL - 6

SP - 475

EP - 479

JO - Alcohol

JF - Alcohol

SN - 0741-8329

IS - 6

ER -