Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets

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Bibtex

@article{53150406c28348b29fdaa23a58d37a44,
title = "Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets",
abstract = "The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.",
keywords = "ethanol, phospholipase C, serotonin, phosphatidic acid, platelets",
author = "Per Simonsson and Istvan Ferencz and Christer Alling",
year = "1989",
doi = "10.1016/0376-8716(89)90081-1",
language = "English",
volume = "24",
pages = "169--174",
journal = "Drug and Alcohol Dependence",
issn = "1879-0046",
publisher = "Elsevier",
number = "2",

}