Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets

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Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets. / Simonsson, Per; Ferencz, Istvan; Alling, Christer.

In: Drug and Alcohol Dependence, Vol. 24, No. 2, 1989, p. 169-174.

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T1 - Ethanol stimulates basal and serotonin-induced formation of [32P]phosphatidic acid in human platelets

AU - Simonsson, Per

AU - Ferencz, Istvan

AU - Alling, Christer

PY - 1989

Y1 - 1989

N2 - The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.

AB - The addition of serotonin to preparations of 32P-labelled human platelets resulted in a time- and dose-dependent hydrolysis of [32P]phosphatidylinositol 4,5-bisphosphate (PIP2) and formation of [32P]phosphatidic acid (PA). This response was inhibited by the serotonin2 receptor antagonist ritanserin, indicating that the stimulation was mediated via the serotonin2 receptor. The addition of 50-150 mM of ethanol prior to stimulation with 10(-5) M serotonin resulted in an increased accumulation of [32P]PA, but had no effect on [32P]PIP2. Ethanol stimulated [32P]PA formation at all serotonin concentrations studied (10(-7)-10(-5) M). Furthermore, in the absence of serotonin, ethanol increased basal [32P]PA formation.

KW - ethanol

KW - phospholipase C

KW - serotonin

KW - phosphatidic acid

KW - platelets

U2 - 10.1016/0376-8716(89)90081-1

DO - 10.1016/0376-8716(89)90081-1

M3 - Article

VL - 24

SP - 169

EP - 174

JO - Drug and Alcohol Dependence

JF - Drug and Alcohol Dependence

SN - 1879-0046

IS - 2

ER -