Etiology of diarrhea in young children in Denmark: a case-control study

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Etiology of diarrhea in young children in Denmark : a case-control study. / Olesen, Bente; Neimann, Jacob; Böttiger, Blenda; Ethelberg, Steen; Schiellerup, Peter; Jensen, Charlotte; Helms, Morten; Scheutz, Flemming; Olsen, Katharina E P; Krogfelt, Karen; Petersen, Eskild; Mølbak, Kåre; Gerner-Smidt, Peter.

In: Journal of Clinical Microbiology, Vol. 43, No. 8, 08.2005, p. 3636-3641.

Research output: Contribution to journalArticle

Harvard

Olesen, B, Neimann, J, Böttiger, B, Ethelberg, S, Schiellerup, P, Jensen, C, Helms, M, Scheutz, F, Olsen, KEP, Krogfelt, K, Petersen, E, Mølbak, K & Gerner-Smidt, P 2005, 'Etiology of diarrhea in young children in Denmark: a case-control study', Journal of Clinical Microbiology, vol. 43, no. 8, pp. 3636-3641. https://doi.org/10.1128/JCM.43.8.3636-3641.2005

APA

Olesen, B., Neimann, J., Böttiger, B., Ethelberg, S., Schiellerup, P., Jensen, C., Helms, M., Scheutz, F., Olsen, K. E. P., Krogfelt, K., Petersen, E., Mølbak, K., & Gerner-Smidt, P. (2005). Etiology of diarrhea in young children in Denmark: a case-control study. Journal of Clinical Microbiology, 43(8), 3636-3641. https://doi.org/10.1128/JCM.43.8.3636-3641.2005

CBE

Olesen B, Neimann J, Böttiger B, Ethelberg S, Schiellerup P, Jensen C, Helms M, Scheutz F, Olsen KEP, Krogfelt K, Petersen E, Mølbak K, Gerner-Smidt P. 2005. Etiology of diarrhea in young children in Denmark: a case-control study. Journal of Clinical Microbiology. 43(8):3636-3641. https://doi.org/10.1128/JCM.43.8.3636-3641.2005

MLA

Vancouver

Author

Olesen, Bente ; Neimann, Jacob ; Böttiger, Blenda ; Ethelberg, Steen ; Schiellerup, Peter ; Jensen, Charlotte ; Helms, Morten ; Scheutz, Flemming ; Olsen, Katharina E P ; Krogfelt, Karen ; Petersen, Eskild ; Mølbak, Kåre ; Gerner-Smidt, Peter. / Etiology of diarrhea in young children in Denmark : a case-control study. In: Journal of Clinical Microbiology. 2005 ; Vol. 43, No. 8. pp. 3636-3641.

RIS

TY - JOUR

T1 - Etiology of diarrhea in young children in Denmark

T2 - a case-control study

AU - Olesen, Bente

AU - Neimann, Jacob

AU - Böttiger, Blenda

AU - Ethelberg, Steen

AU - Schiellerup, Peter

AU - Jensen, Charlotte

AU - Helms, Morten

AU - Scheutz, Flemming

AU - Olsen, Katharina E P

AU - Krogfelt, Karen

AU - Petersen, Eskild

AU - Mølbak, Kåre

AU - Gerner-Smidt, Peter

PY - 2005/8

Y1 - 2005/8

N2 - Infectious gastroenteritis is one of the most common diseases in young children. To clarify the infectious etiology of diarrhea in Danish children less than 5 years of age, we conducted a 2-year prospective case-control study. Stools from 424 children with diarrhea and 870 asymptomatic age-matched controls were examined, and their parents were interviewed concerning symptoms. Rotavirus, adenovirus, and astrovirus were detected by enzyme-linked immunosorbent assay, and norovirus and sapovirus were detected by PCR. Salmonella, thermotolerant Campylobacter, Yersinia, Shigella, and Vibrio spp. were detected by standard methods. Shiga toxin-producing (STEC), attaching-and-effacing (A/EEC), enteropathogenic (EPEC), enterotoxigenic, enteroinvasive, and enteroaggregative Escherichia coli were detected by using colony hybridization with virulence gene probes and serotyping. Parasites were detected by microscopy. Overall, a potential pathogen was found in 54% of cases. More cases than controls were infected with rotavirus, Salmonella, norovirus, adenovirus, Campylobacter, sapovirus, STEC, classical EPEC, Yersinia, and Cryptosporidium strains, whereas A/EEC, although common, was not associated with illness. The single most important cause of diarrhea was rotavirus, which points toward the need for a childhood vaccine for this pathogen, but norovirus, adenovirus, and sapovirus were also major etiologies. Salmonella sp. was the most common bacterial pathogen, followed by Campylobacter, STEC, Yersinia, and classical EPEC strains. A/EEC not belonging to the classical EPEC serotypes was not associated with diarrhea, underscoring the importance of serotyping for the definition of EPEC.

AB - Infectious gastroenteritis is one of the most common diseases in young children. To clarify the infectious etiology of diarrhea in Danish children less than 5 years of age, we conducted a 2-year prospective case-control study. Stools from 424 children with diarrhea and 870 asymptomatic age-matched controls were examined, and their parents were interviewed concerning symptoms. Rotavirus, adenovirus, and astrovirus were detected by enzyme-linked immunosorbent assay, and norovirus and sapovirus were detected by PCR. Salmonella, thermotolerant Campylobacter, Yersinia, Shigella, and Vibrio spp. were detected by standard methods. Shiga toxin-producing (STEC), attaching-and-effacing (A/EEC), enteropathogenic (EPEC), enterotoxigenic, enteroinvasive, and enteroaggregative Escherichia coli were detected by using colony hybridization with virulence gene probes and serotyping. Parasites were detected by microscopy. Overall, a potential pathogen was found in 54% of cases. More cases than controls were infected with rotavirus, Salmonella, norovirus, adenovirus, Campylobacter, sapovirus, STEC, classical EPEC, Yersinia, and Cryptosporidium strains, whereas A/EEC, although common, was not associated with illness. The single most important cause of diarrhea was rotavirus, which points toward the need for a childhood vaccine for this pathogen, but norovirus, adenovirus, and sapovirus were also major etiologies. Salmonella sp. was the most common bacterial pathogen, followed by Campylobacter, STEC, Yersinia, and classical EPEC strains. A/EEC not belonging to the classical EPEC serotypes was not associated with diarrhea, underscoring the importance of serotyping for the definition of EPEC.

KW - Case-Control Studies

KW - Child, Preschool

KW - Diarrhea/etiology

KW - Escherichia coli/isolation & purification

KW - Humans

KW - Infant

KW - Infant, Newborn

KW - Prospective Studies

KW - Rotavirus/isolation & purification

U2 - 10.1128/JCM.43.8.3636-3641.2005

DO - 10.1128/JCM.43.8.3636-3641.2005

M3 - Article

C2 - 16081890

VL - 43

SP - 3636

EP - 3641

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 1098-660X

IS - 8

ER -