Evaluation of assimilatory sulphur metabolism in Caldicellulosiruptor saccharolyticus

Research output: Contribution to journalArticle

Abstract

Caldicellulosiruptor saccharolyticus has gained reputation as being among the best microorganisms to produce
H2 due to possession of various appropriate features. The quest to develop an inexpensive cultivation
medium led to determine a possible replacement of the expensive component cysteine, i.e. sulphate.
C. saccharolyticus assimilated sulphate successfully in absence of a reducing agent without releasing
hydrogen sulphide. A complete set of genes coding for enzymes required for sulphate assimilation were
found in the majority of Caldicellulosiruptor species including C. saccharolyticus. C. saccharolyticus displayed
indifferent physiological behaviour to source of sulphur when grown under favourable conditions
in continuous cultures. Increasing the usual concentration of sulphur in the feed medium increased substrate
conversion. Choice of sulphur source did not affect the tolerance of C. saccharolyticus to high partial
pressures of H2. Thus, sulphate can be a principle sulphur source in an economically viable and more sustainable
biohydrogen process using C. saccharolyticus.

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Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology

Keywords

  • Caldicellulosiruptor saccharolyticus Biohydrogen Sulphate assimiliation
Original languageEnglish
Pages (from-to)677-685
JournalBioresource Technology
Volume169
Publication statusPublished - 2014
Publication categoryResearch
Peer-reviewedYes