Evaluation of Drug Exposure and Metabolism in Locust and Zebrafish Brains Using Mass Spectrometry Imaging

Research output: Contribution to journalArticle

Abstract

Studying how and where drugs are metabolized in the brain is challenging. In an entire organism, peripheral metabolism produces many of the same metabolites as those in the brain, and many of these metabolites can cross the blood-brain barrier from the periphery, thus making the relative contributions of hepatic and brain metabolism difficult to study in vivo. In addition, drugs and metabolites contained in ventricles and in the residual blood of capillaries in the brain may overestimate drugs' and metabolites' concentrations in the brain. In this study, we examine locusts and zebrafish using matrix assisted laser desorption ionization mass spectrometry imaging to study brain metabolism and distribution. These animal models are cost-effective and ethically sound for initial drug development studies.

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Authors
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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology
  • Pharmaceutical Sciences

Keywords

  • blood-brain barrier, clozapine, drug metabolism, Locust, Mass spectrometry imaging, zebrafish
Original languageEnglish
Pages (from-to)1994-2000
Number of pages7
JournalACS Chemical Neuroscience
Volume9
Issue number8
Publication statusPublished - 2018 Aug 15
Publication categoryResearch
Peer-reviewedYes