Evaluation of simple enzyme kinetics by response surface modelling
Research output: Contribution to journal › Article
The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Number of pages||5|
|Publication status||Published - 1999 Dec 1|