Evaluation of simple enzyme kinetics by response surface modelling

Research output: Contribution to journalArticle

Abstract

The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biocatalysis and Enzyme Technology

Keywords

  • Alcohol dehydrogenase, Cyclohexanone, Enzyme kinetics, Response surface modelling
Original languageEnglish
Pages (from-to)903-907
Number of pages5
JournalBiotechnology Techniques
Volume13
Issue number12
Publication statusPublished - 1999 Dec 1
Publication categoryResearch
Peer-reviewedYes