Evidence for disease-regulated transgene expression in the brain with use of lentiviral vectors.

Research output: Contribution to journalArticle

Abstract

In this study we have developed and validated a novel approach of transgene regulation in the brain. By using lentiviral vectors that incorporate promoters of genes that are up-regulated during different pathological states, we were able to regulate transgene expression in accordance with the disease process. When using a glial fibrillary acidic protein promoter, efficient disease regulation in glial cells was achieved after an excitotoxic lesion or a 6-hydroxydopamine (6-OHDA) lesion. Transgene expression was physiologically regulated and displayed a dose-dependent increase depending on the severity of lesion. Efficient regulation was also achieved in neurons when using a preproenkephlin promoter in 6-OHDA-lesioned rats, allowing combined regulation and targeting. This disease-regulated approach allows control of transgene expression in the brain without the use of inducer molecules and without overexpression of transactivator proteins

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences

Keywords

  • disease-regulated transgene expression, brain, lentiviral vectors
Original languageEnglish
Pages (from-to)58-67
JournalJournal of Neuroscience Research
Volume84
Issue number1
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes